Leyton Orient will play in the FA Cup second round for the first time since the 2015-16 season, and the hosts will be dreaming of a glamour third-round tie should they progress.
The O’s squeezed by Ebbsfleet United in the first round, with Aaron Drinan scoring to guide his side to a 1-0 victory.
Kenny Jackett will be hoping that his team can avoid another exit from a cup competition after suffering elimination from the EFL Trophy in midweek.
A goalless draw with MK Dons saw the midweek encounter decided from the spot, with Orient losing 5-4 on penalties.
Before that fixture, Leyton Orient had won four consecutive home games in all competitions, and they are unbeaten in 90 minutes in their last 11 home matches – an indication of the difficult task that faces Tranmere on Saturday.
Tranmere have enjoyed an element of success in the FA Cup in recent years, and a win at the weekend would guarantee a place in the third round for the fourth successive season.
Micky Mellon’s side edged out Crawley Town in the first round, with Callum McManaman’s strike proving enough to clinch a 1-0 victory.
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Since that result, Tranmere have only lost once in four games and they will be buoyant after coming from behind to win against Bradford City in their last outing.
Levi Sutton gave Bradford the lead, but Rovers turned the contest on its head, when Kieron Morris and Paul Glatzel struck in the second half to ensure Tranmere collected their third victory in five games.
Saturday’s visitors have not played since the Bradford contest, with their last two matches postponed due to damage to the stadium caused by Storm Arwen, which means that Tranmere will be well-rested ahead of the second round fixture