Late last week, coach Stuart Baxter said Leonardo Castro and Dumisani Zuma have long-term injuries and have been ruled out.
The coach also said striker Lebogang Manyama started training last week and is not yet fully fit to play.
There is also doubt on the availability of Reeve Frosler and Nkosingiphile Ngcobo, who were at the Olympics with the South Africa national Under-23 team.
But the good news for Chiefs is the fitness of Khama Billiat, who was an injury concern.
Sunday’s encounter could be a difficult one to predict for fans judging from last season’s events.
After Sundowns beat Chiefs 3-0 in the opening match of the PSL last campaign, Masandawana went on to dominate the domestic league while Amakhosi struggled.
But when the Brazilians were expected to walk over Chiefs in the return fixture of the league, the Soweto giants stunned Sundowns 2-1 at Loftus Versfeld in April.
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That makes Sunday’s match a highly anticipated fixture to see who will prevail and proceed to the next round.
Amakhosi will go into this match high in confidence after beating bitter rivals Orlando Pirates in the Carling Black Label Cup two weeks ago as they face a Sundowns side which were last involved in competitive action in early June.
The current Sundowns technical team will be starting their second season since the departure of Pitso Mosimane while Baxter’s return to Chiefs will be scrutinised if he doesn’t land any silverware.